We demonstrate that Nd3+:ethylene glycol can be used as an amplifier gain medium for a Nd3+:YLF laser. It has been known that Nd3+:liquid is hard to use as a gain medium due to fluorescence quenching. However, we could use Nd3+:ethylene glycol as a gain medium by using a new amplification system, and we also measured the stimulated emission cross section of Nd3+:ethylene glycol. A maximum gain of ∼2 was obtained in our experimental setup, and the stimulated emission cross section of Nd3+:ethylene glycol was measured to be 1.5(±0.5) ×10−19cm2. ©1995 American Institute of Physics.