Effects of combined BDNF and GDNF treatment on cultured dopaminergic midbrain neurons
作者:
Jürgen Sautter,
Morten Meyer,
Christian Spenger,
Rolf Seiler,
Hans Widmer,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 6
页码: 1093-1096
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Dopamine;Graft;Neurotrophic factor;Parkinson's disease;Rat;Tissue culture;Transplantation;Ventral mesencephalon
数据来源: OVID
摘要:
Neural transplantation is an experimental therapy for Parkinson's disease. Pretreatment of fetal donor tissue with neurotrophic factors may improve survival of grafted dopaminergic neurons. Free-floating roller tube cultures of fetal rat ventral mesencephalon were treated with brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), or a combination of both. Dopamine content of the culture medium, the number of tyrosine hydroxylase-immunoreactive neurons, and culture volumes were moderately increased in the BDNF- and GDNF-treated cultures but significantly increased by 6.8-, 3.2- and 2.4- fold, respectively after treatment with the combination of both factors. We conclude that pretreatment of dopaminergic tissue in culture with a combination of BDNF and GDNF may be an effective means to improve the quality of tissue prior to grafting.
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