Crop response to fertilizer nitrogen (N) is dependent upon tillage management. This study was conducted to determine how tillage rotation influences non‐irrigated crop growth, N uptake and yield. The effects of tillage rotation, N rate and N timing schedule on early season dry matter production and N uptake, ear leaf N concentration at silking, and yield of corn [Zea mays(L.) Pioneer 3378] were investigated at Painter, VA, on an Altavista loam (fine‐loamy, mixed, thermic Aquic Hapludult). In 1986, maximum yields achieved in the 6‐year continuous no till (NT) [5.82 Mg/ha] and first year no till (AT) [5.64 Mg/ha] were significantly greater than that of the 6‐year continuous conventional till (CT) [3.67 Mg/ha], but no yield differences were obtained in the drier 1987 season. A higher rate of N fertilizer was required to obtain maximum yield in the first year no till (168 kg N/ha) than in the NT (112 kg N/ha) during 1986. Early 1986 N uptake and growth response with and without N at planting increased in the order CT < AT = NT and AT < CT < NT, respectively, indicating greatest immobilization of soil N occurred in the newly established no till soil. Lack of differences in critical ear leaf N values developed for NT and CT in each year imply that plant norms developed for one tillage system may accurately assess N status of corn grown under different tillage practices.