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An enzyme‐linked immunosorbent assay for deoxynivalenol in wheat, utilizing novel hapten derivatization procedures

 

作者: E. N.Clare Mills,   SandraM. Alcock,   HeatherA. Lee,   MichaelR. A. Morgan,  

 

期刊: Food and Agricultural Immunology  （Taylor Available online 1990）
卷期: Volume 2, issue 3  

页码: 109-118

 

ISSN:0954-0105

 

年代: 1990

 

DOI:10.1080/09540109009354710

 

出版商: Taylor & Francis Group

 

关键词: Deoxynivalenol;enzyme‐linked immunosorbent assay;trichothecene;wheat

 

数据来源: Taylor

 

摘要:

Polyclonal anti‐deoxynivalenol (vomitoxin) antisera was produced in rabbits. The immunogen was synthesized by using acetyl esterase to deacylate 3‐acetyldeoxynivalenol hemiglutarate and coupling the product to bovine serum albumin using the mixed anhydride reaction. The antiserum was very specific for deoxynivalenol in a microtitration plate ELISA that had a limit of detection of 90 pg per well. A simple, rapid extraction procedure was employed to enable the ELISA to be used for analysis of the deoxynivalenol in wheat.

 

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