The two-spotted spider mite,Tetranychus urticaeKoch, was identified as the mite pest attacking cotton in 194 of 195 samples taken from 10 counties in the Mississippi Delta. A pathogenic fungus,Entomophthorasp., was found attackingT. urticae(females only) in 92.8% of the samples. Four species of predaceous mites in the family Phytoseiidae were associated with 33 samples. These species wereAmblyseius fallacis(Garman),Galendromus longipilis(Nesbitt),Galendromus pomi(Parrott), andNeoseiulussp. The sex ratio of the tetranychid mites was 1.58 females to 1 male.