Detailed analysis of chromosomes at pachytene and later stages of meiosis were made in the five species ofSorghumbelonging to the Sub-genusEu-sorghum.Pachytene chromosomes are depicted on the basis of total length, relative length, arm ratio, amount and distribution of heterochromatin and number and position of chromomeres. The characteristics of each of the ten chromosomes in the haploid complement are described in detail in different classes bringing out the similarities and differences amongst the five species. Species have been comparedinter aliaon their total lengths of chromosomes and the differences in lengths both within the species and between them were statistically tested.