Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus
作者:
N.M. McKern,
D.D. Shukla,
O.W. Barnett,
H.J. Vetten,
J. Dijkstra,
L.W. Whittaker,
C.W. Ward,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 33,
issue 3
页码: 121-134
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150241
出版商: S. Karger AG
关键词: Potyvirus;Coat protein;High-performance liquid chromatography;Peanut;Azuki bean;Blackeye cowpea;Soybean
数据来源: Karger
摘要:
The interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. Comparison of the coat proteins by the techniques of amino acid analysis and PAGE was inadequate to distinguish strains from distinct potyviruses. However, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. These data indicate that amino acid sequences of coat proteins of azuki bean mosaic virus, the Type and W strains of blackeye cowpea mosaic virus, three isolates (74, PM, PN) of a potyvirus obtained from soybean in Taiwan, and the Blotch and Mild Mottle strains of peanut stripe virus (PStV) may be very similar to the known sequence of PStV Stripe coat protein. In contrast, peptide profiles of coat proteins from soybean mosaic virus, clover yellow vein virus, bean yellow mosaic virus, potato virus Y, and tobacco etch virus were dissimilar to each other and to the profile of PStV Stripe, suggesting that their coat protein sequences were also quite different. Based on observations of the coat protein structure of many potyviruses, the results suggest that the potyvirus isolates with similar coat proteins are strains of the same potyvirus.
点击下载:
PDF
(2161KB)
返 回