Application of Stains-All for Demarcation of Cement Lines in Methacrylate Embedded Bone
作者:
GruberHelen E.,
MekikianPertchoui,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 4
页码: 181-184
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109966
出版商: Taylor&Francis
关键词: bone;cement lines;Stains-all
数据来源: Taylor
摘要:
Cement lines provide a record of sites of past remodeling buried in the matrix of bone. A method is reported for application of Stains-all, a cationic carbocyanine dye, for demarcation of cement lines in bone. The method, which is simple, works well for both glycol methacrylate and methyl methacrylate undemineralized embedments and produces good concomitant staining of cytoplasm and nuclei of osteoblasts, osteoclasts and marrow cells.
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