AbstractThe metabolism of 1,2‐dimethylhydrazine (DMH) was investigated in isolated perfused livers excised from Sprague‐Dawley rats fed four semisynthetic diets over two generations. The diets were varied within normal physiological limits, without producing specific deficiencies. Diet was shown to have an influence on the growth of animals and on the cholesterol content andß‐glucuronidase activity in the blood serum. However, diet did not influence the rate of metabolism of DMH or the concentration of metabolites. (Nutr Cancer 6, 181–186, 1984)