Galline, a protamine from rooster sperm nuclei, has already been separated into its components, G‐I‐G‐VIII. The amino acid composition of the homogeneously purified fraction G‐I is determined decisively to be Arg11, Ser2, Gly3, Val1and Tyr2, and the molecular weight is 2,908 as hydrochloride. The complete amino acid sequence was deduced as follows from the results of analyses of the tryptic and chymotryptic peptides: H‐Ser‐Gly‐Gly‐Val‐Arg‐Arg‐Arg‐Arg‐Tyr‐Gly‐Ser‐Arg‐Arg‐Arg‐Arg‐Arg‐Arg‐Arg‐Tyr‐OH.Another purified fraction, G‐V, has the amino acid composition, Arg24, Thr1, Ser8, Gly3, Ala2, Pro2and Tyr2as described previously, and the molecular weight 6,274 as hydrochloride. The complete amino acid sequence was established by combining analytical results of tryptic, chymotryptic and thermolysin peptides as well as those of carboxypeptidase and leucine aminopeptidase digestion as follows: H‐Ala‐Arg‐Tyr‐Arg‐Ser‐Gly‐Arg‐Ser‐Arg‐Ser‐Arg‐Arg‐Thr‐Arg‐Arg‐Arg‐Arg‐Ser‐Pro‐Arg