Histochemistry of carbohydrates in the epithelium lining the bulbourethral gland of the rat
作者:
Azuma Tsukise,
Yukio Sugawa,
Kazuyorí Yamada,
期刊:
Cells Tissues Organs
(Karger Available online 1979)
卷期:
Volume 105,
issue 4
页码: 529-538
ISSN:1422-6405
年代: 1979
DOI:10.1159/000145160
出版商: S. Karger AG
关键词: Histochemistry;Carbohydrates;Epithelium;Bulbourethral gland;Rat
数据来源: Karger
摘要:
The histochemistry of carbohydrates has been studied in the epithelium lining the bulbourethral gland of the rat by means of light- and electron-microscopic methods. The results obtained show that the cytoplasms of the epithelial cells contain neutral and acidic carbohydrates. The neutral carbohydrates exhibited positive reactions with periodic acid-Schiff and periodic acid-thiosemicarbazide-silver proteinate, whereas the acidic carbohydrates reacted positively with alcian blue (AB; pH 1.0 and 2.5) and dialyzed iron. Most neutral carbohydrates were found to be glycoproteins localized within the secretory granules. The acidic carbohydrates consist of at least two types, AB (pH 1.0)-reactive sulfated and AB (pH 2.5)-reactive nonsulfated carbohydrates; most nonsulfated carbohydrates were determined to be sialic acid. The acidic carbohydrates were also localized within the secretory granules.
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