Fluorimetric Determination of Plasma Cortisol in Chronic Renal Failure
作者:
AnwarS. Mohamed,
FardosA. Mohamed,
HosnyA. Hassan,
NadiaM. Selim,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 8
页码: 1265-1273
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708064607
出版商: Taylor & Francis Group
关键词: Cortisol;Fluorescence;Renal Failure
数据来源: Taylor
摘要:
A fluorimetric method for the determination of plasma cortisol has been studied in detail. The method is based upon reaction with ethanol-sulphuric acid reagent and measurement of the produced fluorescence at exc. 470 and em. 550 nm. Some modifications were suggested regarding extraction solvent and time of the reaction. Under the proposed conditions, the method is rapid and a sensitivity of 1.5 μg/100 ml was attained. It was applied on 29 patients with chronic renal failure and 12 normal subjects. Cortisol levels at 8 AM in patients on either haemodialysis (27.05 ± 8.94 μg/100 ml) or under conservative treatment (45.13 ± 10.68 μg/100 ml) were significantly higher (P < 0.001) than control group (16.12 ± 3.45 μg/100 ml).
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