Retention model for the resolved enantiomers of felodipine on chiral‐AGP using micellar mobile phases
作者:
Dan Haupt,
Curt Pettersson,
Douglas Westerlund,
期刊:
Chirality
(WILEY Available online 1995)
卷期:
Volume 7,
issue 1
页码: 23-27
ISSN:0899-0042
年代: 1995
DOI:10.1002/chir.530070105
出版商: Alan R. Liss, Inc.
关键词: felodipine;retention model;micellar mobile phases;chiral resolution;CHIRAL‐AGP
数据来源: WILEY
摘要:
AbstractA retention model for the chiral separation of an uncharged solute, felodipine, on CHIRAL‐AGP, using a micellar mobile phase is proposed. The model assumes the presence of two stereoselective sites and each enantiomer was found to interact with different sites. Addition of a chiral aliphatic alcohol, (+)‐(S)‐2‐octanol, preferentially interacted with the binding site for (−)‐(S)‐felodipine. The monomeric form of the micellar agent (Tween® 20) competed with the enantiomers for the adsorption sites, and the formation of a 1:1 complex between the enantiomers and the micelles was assumed. The retention of the solutes was effectively controlled by adding small quantities (<1.63 × 10−3M) of the nonionic detergent Tween 20 to the mobile phase. Baseline separation was achieved by addition of 1.0 mMn‐octylamine to the mobile phase; 8.14 × 10−4MTween 20 in phosphate buffer pH 7.0. The separation factor (α = 1.74) was unaffected by the detergent concentration in the presence of 1.0 mMn‐octylamin
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