PROTEIN SEPARATION BY CROSS-AXIS COIL PLANET CENTRIFUGE WITH TWO DIFFERENT POSITIONS OF ECCENTRIC COIL ASSEMBLIES USING POLYETHYLENE GLYCOL-DEXTRAN SOLVENT SYSTEM
作者:
Kazufusa Shinomiya,
Shingo Hirozane,
Yozo Kabasawa,
Yoichiro Ito,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 7
页码: 1119-1129
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101513
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Countercurrent chromatography (CCC) of proteins was performed by the cross-axis coil planet centrifuge (cross-axis CPC) using polyethylene glycol (PEG) - dextran biphasic systems. A pair of eccentric coil assemblies was mounted at two different positions (off-center and central) on the rotary frame. The best separation was obtained from a two-phase solvent system composed of 4.0% (w/w) PEG 8000 - 5.0% (w/w) dextran T500 in 5 mM potassium phosphate buffer (pH 7.0) and 3 M sodium chloride using the upper PEG-rich phase as the mobile phase. The off-center column position yielded substantially better peak resolution between lysozyme and myoglobin than that obtained from the central position under otherwise identical conditions.
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