首页   按字顺浏览 期刊浏览 卷期浏览 Chromosome Analysis in Suspension Culture ofVigna SinensisVar. Black andPisum SativumL.
Chromosome Analysis in Suspension Culture ofVigna SinensisVar. Black andPisum SativumL.

 

作者:

 

期刊: Caryologia  (Taylor Available online 1979)
卷期: Volume 32, issue 4  

页码: 419-424

 

ISSN:0008-7114

 

年代: 1979

 

DOI:10.1080/00087114.1979.10796805

 

出版商: Taylor & Francis

 

数据来源: Taylor

 

摘要:

The study on the extent of chromosomal variations in suspension cultures initiated from hypocotyl segments ofPisum sativumvar. Arkel andVigna sinensisvar. black reveals aberrations like inhibition of cell plate formation, spindle abnormalities, chromosomal breakage, polyploidy and aneuploidy. Such aberrations are frequently observed mostly in aneuploid cells and their frequencies increase with the number of passages. Asynchronous behaviour of daughter nuclei resulting in multinucleate cells is observed more frequently in cultured tissues of P.sativumL. than inVigna sinensisvar. black. In progressively older passages ofVigna sinensisvar. black diploid cell populations gradually increase more rapidly than the tetraploid and aneuploid cells, indicating the selective advantage of diploid cells over others. By contrast in progressively old passages ofPisum sativumL. the frequencies of cells with 11 and 12 chromosomes are high. In the resulting cell population a range from 7 to 14 chromosomes could be noticed indicating instability. Anaphase bridges occur in almost the same frequency in both the species. Some other features of the chromosomal variations have been described.

 

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