Expression of mRNAs encoding BDNF and its receptor in adult rat hypothalamus
作者:
Frédéeric Marmigere,
Florence Rage,
Lucia Tapia-Arancibia,
Sandor Arancibia,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 6
页码: 1159-1163
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: BDNF;DIG-labeled-cRNA;Hypothalamus;Multiple promoters;Trk receptors
数据来源: OVID
摘要:
WE used a digoxigenin-UTP-labeled cRNA probe within situhybridization and Northern blot analysis to investigate the localization of brain derived neurotrophic factor (BDNF) mRNA and expression of its different transcripts in adult rat hypothalamus. As the BDNF gene is under the control of alternative multiple promoters, which provide tissue-specific gene expression, we studied whether these transcripts were expressed in adult hypothalamus. Our data revealed two novel sources of hypothalamic BDNF mRNA: the supraoptic and periventricular nuclei. In addition, we observed the expression of transcripts from exons I, II and III as well as the presence of 1.6 and 4.2 kb BDNF mRNAs. Finally, our findings confirmed expression of mRNA encoding neurotrophins receptors in the hypothalamus.
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