Storage of samples infected with lettuce or cucumber mosaic viruses prior to testing by ELISA
作者:
C. M. WARD,
D. G. A. WALKEY,
KATHLEEN PHELPS,
期刊:
Annals of Applied Biology
(WILEY Available online 1987)
卷期:
Volume 110,
issue 1
页码: 89-95
ISSN:0003-4746
年代: 1987
DOI:10.1111/j.1744-7348.1987.tb03235.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYMethods of processing and storing lettuce mosaic and cucumber mosaic virus infected tissues prior to ELISA have been examined. Virus antigen detection was highest in samples homogenised in phosphate inoculation buffer (1% K2HPO4, 0.1% Na2SO3) and stored at either — 20°C or — 196°C. Detection was poor in samples homogenised in phosphate buffered saline prior to storage and in samples of all treatments stored at 4°C. Freeze‐dried leaf segments retained a high concentration of antigen, as did freeze‐dried homogenates of samples prepared in inoculation buffer.Higher levels of antigen were detected in samples stored as whole leaves at 4°C for 24 h before processing, than in samples stored as leaf segments during
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