Enzymatic Determination of Ammonia in Lake Water Using a Semi-Automatic Analyser
作者:
S. Hashimoto,
H.Y. Sun,
A. Otsuki,
S. Kawakami,
F. Murata,
M. Yokomizo,
期刊:
International Journal of Environmental Analytical Chemistry
(Taylor Available online 1992)
卷期:
Volume 48,
issue 3-4
页码: 155-161
ISSN:0306-7319
年代: 1992
DOI:10.1080/03067319208027396
出版商: Taylor & Francis Group
关键词: Ammonia determination;enzymatic method;semi-automatic analyser;lake water;glutamate dehydrogenase
数据来源: Taylor
摘要:
An enzymatic method was developed for the determination of ammonia concentrations in lake water. Lake water samples containing ammonia were mixed with a glutamate dehydrogenase (GIDH), reduced nicotinamide adenine dinucleotide phosphate (NADPH) and 2-oxoglutarate. The decrease in the absorbance intensity caused by the disappearance of NADPH by this reaction was measured at 340 nm. There was a linear relationship (r= 0.9997) between peak height and ammonia concentration over the range 0–29 μM. The detection limit was 0.29 μM for a sample volume of 250 μl. Interference of amino acids and urea at concentrations of 50 mg l−1was negligible. Good agreement was found between the enzymatic method and indophenol blue colorimetry.
点击下载:
PDF (316KB)
返 回