The relationship between accommodative fluctuations and perimetric sensitivity was investigated in a group of 10 clinically normal emmetropic subjects using the Dicon AP3000 computer‐assisted perimeter at a bowl luminance of 10 asb. Accommodative microfluctuations were attenuated with the antimuscarinic drug cyclopentolate HCL 1% and the concomitant mydriasis was matched on a separate trial using the sympathomimetic phenylephrine HCI 10%. Saline 0.9% was used as the control. Accommodative microfluctuations were found to play a minor role in determining the magnitude of sensitivity out to an eccentricity of 50; between 50and 27.50, the effect of microfluctuations was masked by the mydriasis produced by the drugs used in the study. The range of sensitivity values at a given location were reduced when accommodative fluctuations were minimize