Localization of heterochromatin in human chromosomes
作者:
Frances E. Arrighi,
T.C. Hsu,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1971)
卷期:
Volume 10,
issue 2
页码: 81-86
ISSN:1424-8581
年代: 1971
DOI:10.1159/000130130
出版商: S. Karger AG
数据来源: Karger
摘要:
Heterochromatic regions in chromosomes of man, mainly at the centromeric areas, can be demonstrated with consistency using a special staining procedure. This procedure includes treatments of cytological preparations with HCl, RNase, NaOH (denaturing DNA), and renaturing DNA in a saline citrate solution at 65°C. The heterochromatic regions can be observed after staining with Giemsa. These regions are believed to be composed of constitutive heterochromatin. Facultative heterochromatin regions, e.g., the repressed X in female cells, do not respond to this series of treatments
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