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A Biosensor for ADP with Internal Substrate Amplification

 

作者: U. Wollenberger,   F. Schubert,   F. Scheller,   B. Danielsson,   K. Mosbach,  

 

期刊: Analytical Letters  （Taylor Available online 1987）
卷期: Volume 20, issue 5  

页码: 657-668

 

ISSN:0003-2719

 

年代: 1987

 

DOI:10.1080/00032718708062919

 

出版商: Taylor & Francis Group

 

关键词: Multienzyme electrode;enzymatic amplification

 

数据来源: Taylor

 

摘要:

An enzyme electrode was constructed from an oxygen electrode and a layer incorporating four enzyme systems for the sensitive determination of ADP and ATP. The cofactor is cycled between pyruvate kinase and hexokinase under formation of pyruvate which is detected by the coimmobilized, sequentially acting enzymes lactate dehydrogenase and lactate monooxygenase. The enzymatic recycling results in a 220-fold increased sensitivity to ADP compared to the unamplified reaction.

 

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