Recombinant PDGF from Lower Vertebrates: Receptor Binding and Immunochemical Analysis with Metabolically Labeled Growth Factor
作者:
WangChiayeng,
ShamahSteven M.,
StilesCharles D.,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 7,
issue 4
页码: 279-288
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209046410
出版商: Taylor&Francis
关键词: receptors;frog;biosynthesis;baculovirus;antibodies
数据来源: Taylor
摘要:
AbstractWe used a baculovirus vector/insect host cell system to express cDNA clones of PDGF A genes from mouse and frog (Xenopus laevis). The insect host cells process PDGF A subunits from either frogs or mice into biologically active AA homodimers with yields in the range of 0.5-1.0 mg/liter of culture medium. The recombinant PDGFs can be metabolically labeled with35S-cysteine for use in radioreceptor and radioimmunoassays. Neutralizing polyclonal antisera can be raised against the mouse and frog PDGFs. These antisera are markedly species-specific in action. However, in radioreceptor binding assays and bioassays for mitogenic activity, human, mouse and frog PDGF AA homodimers occupy and activate murine PDGF receptors with equal efficiency.
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