Rapid Identification of Viable Bacterial Spores Using a Fluorescence Method
作者:
SharmaDinesh K.,
PrasadD. N.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 1
页码: 27-29
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110001
出版商: Taylor&Francis
关键词: viable-nonviable spores;germination-like changes;differentiation;UHT;conventional plating
数据来源: Taylor
摘要:
A method has been devised to differentiate viable and nonviable bacterial spores.“Germination-like”changes are initiated in spores with performic acid and lysozyme. The germinated spores are stained with aqueous acridine orange, a fluorescent dye. Nonviable spores fluoresce lemon-green and viable spores orange-red. It is proposed that with the use of a membrane filter resistant to performic acid and lysozyme, the method may be used for spore enumeration in foods in about 4 hr compared to conventional plating methods, which usually require up to 72 hr.
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