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Rapid Identification of Viable Bacterial Spores Using a Fluorescence Method

 

作者: SharmaDinesh K.,   PrasadD. N.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1992)
卷期: Volume 67, issue 1  

页码: 27-29

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110001

 

出版商: Taylor&Francis

 

关键词: viable-nonviable spores;germination-like changes;differentiation;UHT;conventional plating

 

数据来源: Taylor

 

摘要:

A method has been devised to differentiate viable and nonviable bacterial spores.“Germination-like”changes are initiated in spores with performic acid and lysozyme. The germinated spores are stained with aqueous acridine orange, a fluorescent dye. Nonviable spores fluoresce lemon-green and viable spores orange-red. It is proposed that with the use of a membrane filter resistant to performic acid and lysozyme, the method may be used for spore enumeration in foods in about 4 hr compared to conventional plating methods, which usually require up to 72 hr.

 

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