Three Types of Naturally Occurring Modified Lipoproteins Induce Intracellular Lipid Accumulation Due to Lipoprotein Aggregation
作者:
V. Tertov,
A. Orekhov,
I. Sobenin,
Z. Gabbasov,
E. Popov,
A. Yaroslavov,
V. Smirnov,
期刊:
Circulation Research
(OVID Available online 1992)
卷期:
Volume 71,
issue 1
页码: 218-228
ISSN:0009-7330
年代: 1992
出版商: OVID
关键词: atherosclerosis;cell cultures;modified lipoproteins;lipoprotein aggregation;lipid accumulation
数据来源: OVID
摘要:
Low density lipoprotein (LDL) from patients with coronary atherosclerosis and diabetes mellitus as well as in vitro desialylated LDL, glycosylated LDL, and lipoprotein (a) caused a twofold to fourfold rise in cholesteryl ester in cultured human blood monocytes and intimal smooth muscle cells isolated from normal aorta. Native LDL from healthy subjects failed to induce intracellular lipid accumulation. We have demonstrated by laser correlative photometry and gel filtration chromatography that in vivo and in vitro modified lipoproteins form aggregates under cell culture conditions. The degree of modified lipoprotein aggregation directly correlated with the ability of these lipoproteins to elevate the cholesteryl ester content of cultured cells. Modified lipoprotein aggregates isolated by gel filtration induced a threefold to fivefold elevation in cellular cholesteryl ester content. Aggregates of125I-modified LDL were taken up and degraded fivefold to sevenfold more effectively as compared with nonaggregated lipoproteins. The uptake and degradation of125I-labeled aggregates were strongly inhibited by unlabeled aggregates, latex beads, and cytochalasin B but not by native or acetylated LDL. These data indicate that uptake of lipoprotein aggregates occurred by phagocytosis. Obtained results suggest that modified lipoprotein aggregation may be the key condition for lipid accumulation.
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