Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations
作者:
E. B. Hunziker,
W. Herrmann,
L. M. Cruz‐Orive,
A. L. Arsenault,
期刊:
Journal of Electron Microscopy Technique
(WILEY Available online 1989)
卷期:
Volume 11,
issue 1
页码: 9-15
ISSN:0741-0581
年代: 1989
DOI:10.1002/jemt.1060110103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Calcifying cartilage;Mineral crystals;Matrix vesicles
数据来源: WILEY
摘要:
AbstractBiological mineralization is a cell‐mediated process which is believed to be triggered by a “nucleating agent.” Various matrix structures, such as matrix vesicles, collagen fibrils and macromolecules, have been claimed to be the source of this substance, since these components have been found by transmission electron microscopy (TEM) of thin sections to be associated with early mineral crystals. Systematic image analysis of the relationships revealed in electron micrographs between specific matrix components and early mineral deposits has shown that unequivocal image interpretation is not possible. This is due principally to the problems posed by overprojection and truncation phenomena, since the structures being analyzed lie within the same dimensional range as thin section thickness. Various examples are illustrated and discussed. The site at which mineral crystals are initially laid down thus cannot be identified with any matrix structure using thin section TEM. Possible technical approaches to resolve this problem of image analysis are disc
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