Resolution of Nomifensine Enantiomers on Selected Achiral and Chiral Stationary Phases Using High Performance Liquid Chromatography
作者:
Mohamed M. Hefnawy,
James T. Stewart,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 1
页码: 159-171
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542605
出版商: Taylor & Francis Group
关键词: Nomifensine;HPLC;β-CD;inclusion and brush-type stationary phases;chiral mobile phase additive
数据来源: Taylor
摘要:
The separation of the enantiomers of nomifensine maleate, a psychotropic drug, were investigated using achiral and chiral stationary phases Three stationary phases were studied for their ability to separate the enantiomers: reversed phase cyanopropyl with a chiral mobile phase additive; inclusion (cellulose tris 3,5-dimethylphenyl carbonate (chiracel OD); and brush/pirkle type (S-tert-leucine, (R)-1-(α-naphthyl) ethylamine (Sumichiral OA-4700). Changes in mobile phase composition were studied to determine the effect of solvents on the separation of the enantiomers. Baseline separations were achieved for nomifensine enantiomers under isocratic conditions on the cyanopropyl column with 90:10 v/v 0.1% triethylamine-acetonitrile pH 4.0 (adjusted by trifluoroacetic acid) containing 10 mM β-cyclodextrin, on a Chiracel OD column using hexane-absolute methanol (96:4 v/v), and on a Sumichiral OA-4700.column using hexane-dichloroethane-absolute ethanol-trifluoroacetic acid (100:12.5:8.5:0.1v/v/v/v). The flow rates were 0.8 mL/min with detection at 292 nm.
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