Microbial invasion and growth in amniotic fluid play an important role in infectious perinatal morbidity and mortality. In order to determine the influence of amniocentesis (ACT) on intra-amniotic infection development, we performed a study of 239 complete microbiological examinations of amniotic fluid specimens obtained by serial interventions. In 1 case (0.42%), during the second procedure, Staphylococcus epidermidis was discovered. Neither spontaneous abortion nor preterm labor were provoked by the procedure. We can conclude that ACT has been confirmed as a safe and successful intrauterine intervention if it is made in a proper manner.