A New Disposable Biosensor for the Accurate and Sensitive Detection of Ethylenebis(Dithiocarbamate) Fungicides
作者:
Thierry Noguer,
Adriana Gradinaru,
Anton Ciucu,
Jean-Louis Marty,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 9
页码: 1723-1738
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542928
出版商: Taylor & Francis Group
关键词: biosensor;disposable;inhibition;ethylenebis(dithiocarbamate);fungicides;nabam;aldehyde dehydrogenase;NADH oxidase;entrapment
数据来源: Taylor
摘要:
A disposable bienzymic sensor for the reliable determination of dithiocarbamate fungicides was developed. Aldehyde dehydrogenase and a highly stable NADH oxidase were immobilized on the surface of a disposable Pt-sputtered carbon paste screen printed electrode by using photocrosslinkable poly(vinylalcohol) bearing styrylpyridinium groups. Due to the very poor solubility of ethylenebis(dithiocarbamates), a preliminary transformation of these compounds in a highly soluble sodium form was carried out using EDTA, disodium form. The advantages of performing this previous step was both to solubilize the compounds and to use a commercial disodium ethylenebis(dithiocarbamate), i.e. nabam, as calibration compound. The sensor was used for the determination of EDTA/Na2-transformed zineb and the results showed a good correlation with those achieved with nabam. As low as 8 ppb of pesticide has been detected in these first experiments, whereas the detection limit of the recommended spectrophotometric method is about 400 ppm.
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