Preliminary Evaluation of Mixed Dyes for Fingerprinting Non-Fluorescent Bacteria
作者:
DennisC. Shelly,
JohnM. Quarles,
IsiahM. Warner,
期刊:
Analytical Letters
(Taylor Available online 1981)
卷期:
Volume 14,
issue 13
页码: 1111-1124
ISSN:0003-2719
年代: 1981
DOI:10.1080/00032718108081450
出版商: Taylor & Francis Group
关键词: Rapid Scanning Fluorometry;Video Fluorometry;Bacterial Fingerprinting;Fluorescent Dyes
数据来源: Taylor
摘要:
A rapid scanning fluorometer is used to obtain fluorescence spectral data characteristic of bacteria which do not readily produce fluorescent pigments. The data is generated in the form of an array of fluorescence intensity as a function of multiple excitation and emission wavelengths. Fluorescence spectral properties are introduced into the bacterial specimen using multiple dye mixtures for staining. The characteristic fluorescent fingerprints are believed to occur by preferential adsorption and reaction of bacterial cellular components with the individual dyes of the mixture. The procedure is found to be rapid and reproducible for the bacteria examined.
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