Heparin Neutralization during Collection and Processing of Blood Inhibited by Pyridoxal 5′-Phosphate
作者:
Janjaap van Putten,
Marjo van de Ruit,
Marlène Beunis,
Coenraad Hemker,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1984)
卷期:
Volume 14,
issue 3
页码: 253-261
ISSN:1424-8832
年代: 1984
DOI:10.1159/000215066
出版商: S. Karger AG
关键词: Heparin determination;Heparin neutralization;Pyridoxal phosphate;Heparin monitoring;Platelet inhibition;Blood sampling
数据来源: Karger
摘要:
Spectrophotometric heparin assays are expected to be independent on clotting factors, either activated or nonactivated, but could be sensitive to heparin neutralization during blood collection. Is was shown that more than 200 U of heparin/1 plasma can completely be neutralized during blood processing. Because the heparin neutralization is not constant but dependent on the sample as well as on the type of sample handling, one cannot beforehand compensate and correct the results for possible heparin neutralization. We tested the use of pyridoxal 5’-phosphate (PLP) to prevent heparin neutralization. As the use of PLP in the citrate tube decreased the heparin neutralization to a negligible effect, PLP-citrate tubes are to be preferred for all plasma heparin determination
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