Reproducibility of a commercially available kit utilizing enzyme‐linked immunosorbent assay for determination of aflatoxin in peanut butter
作者:
C. M. Ward,
M. R. A. Morgan,
期刊:
Food Additives & Contaminants
(Taylor Available online 1991)
卷期:
Volume 8,
issue 1
页码: 9-15
ISSN:0265-203X
年代: 1991
DOI:10.1080/02652039109373951
出版商: Taylor & Francis Group
关键词: Aflatoxin;ELISA;reproducibility;peanut butter;kit
数据来源: Taylor
摘要:
The reproducibility of a commercially available kit utilising enzyme‐linked immunosorbent assay (ELISA) for determination of total aflatoxins has been analysed. Peanut butter samples contaminated with aflatoxin at three concentrations (mean 5.8, 11.7 and 24.8 ng/g) were analysed on different occasions using four kits. Coefficients of variation at all concentrations ranged from 2.6% to 13.6% within replicate, 0.3% to 16.2% within plate and 6.7% to 10.2% between kits. Standard curve precision profiles showed a minimum coefficient variation (CV) of 2–4% and a working range (below 10% CV) covering almost the entire standard curve.
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