Purification of bovine P2 myelin protein with bound lipids
作者:
Paolo Riccio,
Francesco Zito,
Anna Fasano,
Grazia Liuzzi,
Francesco Lolli,
Eugenia Polverini,
Paolo Cavatorta,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 12
页码: 2769-2773
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Experimental allergic neuritis;Lipids;Myelin;Myelin basic protein;P2;PNS;Protein conformation
数据来源: OVID
摘要:
THE P2 protein is a neuritogenic, small basic protein present in PNS myelin. It belongs to the family of the cytoplasmic lipid-binding proteins and can be incorporated in lipidic bilayers. P2 has been purified and crystallized only in the lipid-free form. Here we show that the P2 protein can be purified with bound lipids by applying to PNS myelin the same procedure that as used to purify lipid-bound myelin basic protein from CNS myelin. SDS-PAGE showed a single band of 16. 5 kDa, and TLC showed the presence of most of the myelin lipids associated with the protein. Lipid-bound P2 revealed different circular dichroism spectra from the corresponding lipid-free form, indicating that lipids influence P2 conformation
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