Preparation of Human Fibrinogen Free of Plasminogen by Immunoadsorption
作者:
K.H. Luke,
J. Hirsh,
J. Bienenstock,
A. Zipursky,
M. Johnson,
K. Allman,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1972)
卷期:
Volume 1,
issue 3-4
页码: 210-214
ISSN:1424-8832
年代: 1972
DOI:10.1159/000213987
出版商: S. Karger AG
关键词: Fibrinogen;Plasminogen;Immunoadsorption
数据来源: Karger
摘要:
Human fibrinogen was purified free of plasminogen by a simple immunoadsorption technique using an insoluble polymer complex of an anti-plasminogen antiserum cross-linked by ethylchloroformate. The anti-plasminogen antiserum was prepared in guinea pigs by immunization with purified human plasminogen. The fibrinogen so purified was free of plasminogen as demonstrated by immunological and biological assays and did not show changes in clottability, thrombin clotting time and reactivity to plasmin, and is suitable as a reagent for plasmin and anti-plasmin assays for diagnostic purposes.
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