Differentiation of Normal and Neoplastic Cells by Synchronous Fluorescence: Rat Liver Epithelial and Rat Hepatoma Cell Models
作者:
Wendi E. Watts,
Narayana R. Isola,
Donita Frazier,
Tuan Vo-Dinh,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 13
页码: 2583-2594
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542989
出版商: Taylor & Francis Group
关键词: synchronous luminescence;synchronous fluorescence;cancer diagnosis;normal rat liver epithelial;hepatoma cell lines
数据来源: Taylor
摘要:
In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SF) method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval between them. This SF procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differences in the fluorescence emission of the biochemical species of cells from rat tissues. A difference between the fluorescent spectra of the normal rat liver epithelial (RLE) and hepatoma cell lines were detected using synchronous fluorescence. The potential use of SF as a screening tool for cancer diagnosis is discussed.
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