A simple and sensitive LC method for the determination of amino compounds, using carbazole-N-acetyl-N-hydroxy-succinimide (CAHS) as condensation agent, has been developed.A mixture of amines is treated with CAHS, in the presence of triethylamine catalyst in non-aqueous acetonitrile or in the presence of 0.2 M borate buffer at pH 8.0–9.0 in 40% (v/v) of acetonitrile solution, to give quantitative yields of amides. Emission maximum for the derivatized amines is 365 nm (λex335 nm). Studies on derivatization conditions indicate that amines react very fast with CAHS under proposed conditions. The method, in conjunction with a multi-step gradient, offers baseline resolution of common amine derivatives on a reversed-phase C18column.