A NOVEL METHOD FOR PRODUCTION OF MONOCLONAL ANTIBODIES. EVALUATION AND EXPECTATION OF THE RAT LYMPH NODE METHOD IN CELL AND MOLECULAR BIOLOGY
作者:
YOSHIKAZU SADO,
TOHRU OKIGAKI,
期刊:
Cell Biology International
(WILEY Available online 1996)
卷期:
Volume 20,
issue 1
页码: 7-14
ISSN:1065-6995
年代: 1996
DOI:10.1006/cbir.1996.0003
出版商: Blackwell Publishing Ltd
关键词: monoclonal antibody;antipeptide antibody;type IV collagen;basement‐membrane collagen;rat lymph node
数据来源: WILEY
摘要:
AbstractA newly developed method using rat lymph nodes provided us with substantial amounts of high quality monoclonal antibodies. The method has characteristic features, a single injection of an emulsified antigen via the rat's hind footpads is sufficient for immunization. Enlarged medial iliac lymph nodes can be used for cell fusion to produce hybridomas 2 weeks and later after the antigen injection. The production frequency of target hybridomas is about 10 times higher than that of a conventional spleen method. Our current knowledge about the production of monoclonal antipeptide antibodies and monoclonal nephritogenic autoantibodies by the method is described.
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