C-13 NMR Investigation of Cellobiose in the Presence and Absence of β-Glucosidase
作者:
N. Marchettini,
L. Pogliani,
C. Rossi,
S. Ulgiati,
期刊:
Spectroscopy Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 1
页码: 81-86
ISSN:0038-7010
年代: 1987
DOI:10.1080/00387018708082278
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
T1(C-13) NMR measurements of cellobiose in aqueous solutions at pH=5 in the presence and absence of enzyme β-glucosidase detected at T=276°K a fast interaction between cellobiose and the enzyme at C1′α level of the disaccharide molecule. T1(C-13) measurements of equal solutions of cellobiose in the presence and absence of EDTA at the same temperatures suggest also a competitive interaction of cellobiose, at C1′α level, with EDTA complexed with a metal ion. T1 data justify a nearly spherical shape of cellobiose in solution.
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