Detection of Viruses and Bacteria with Piezoelectric Immunosensors
作者:
Bernd König,
Michael Grätzel,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 8
页码: 1567-1585
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308021481
出版商: Taylor & Francis Group
关键词: Piezoelectric Immunosensor;Rotavirus;Acute Diarrhea;Clinical Diagnostics
数据来源: Taylor
摘要:
A reusable piezoelectric immunosensor has been developed for the detection of viruses and bacteria, playing an important role in acute diarrhea in early childhood. Three different methods for antibody immobilization to the gold electrode were tested. Coating the electrode with protein A gave the best results in terms of stability, sensitivity and reproducibility. Worse results were observed when coating the electrode with γ-amino propyltriethoxy silane and polyethyleneimine. Applying an antibody layer via protein A immobilization onto a 10-MHz AT-cut crystal resulted for 1 × 106to 1 × 1010virus cells and for 1 × 106to 1 × 108bacteria in a linear frequency change. The modified crystal was stable for six weeks when stored desiccated at +4°C.
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