AN IMPROVED ASSAY BY HPLC WITH AMPEROMETRIC DETECTION FOR THE DETERMINATION OF PHENTOLAMINE IN PLASMA
作者:
J. Pérez-Urizar,
P. Aguirre-Bañuelos,
G. Castañeda-Hernández,
F.J. Flores-Murrieta,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 4
页码: 557-564
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101472
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
An improved method for the determination of phentolamine in human plasma samples was developed. After being alkalinized, plasma samples (1 mL) were extracted with diethyl ether and then back-extracted with O.1 N HCl. Analyses were carried out on a Novapak C8 column eluted with a mixture of sodium monochloroacetate (pH 3) and acetonitrile (75:25). Amperometric detection was performed by oxidation at 1000 mV, using a glassy carbon electrode against Ag/AgCl. Calibration curves, constructed over a 1 to 30 ng/mL plasma concentration range, were linear (r=0.999). Intra-assay coefficients of variation and accuracy for the determined concentrations were comprised within 7.6–10.9% and 94.0–105.6%, respectively. Inter-assay coefficient of variation and accuracy ranges were 10.4–20.7% and 93.2–102.7%, respectively. The method's detection limit was 0.2 ng/mL, allowing determination of oral phentolamine pharmacokinetics after administration of a 40 mg dose.
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