Seeds of four lisianthus (Eustoma grandiflorum) cultivars were germinated and transplanted into an equal volume of peat, perlite medium without fertilizer amendments. Plants were grown in the greenhouse and watered daily with a modified Hoagland's solution containing 0, 100, 200, or 400 mg N/l in a N experiment, and 0, 75, 150 or 300 mg Ca/l in a Ca experiment. Plants were harvested at the end of 8 weeks. Increasing N concentrations in the nutrient solution decreased stem length and number of flowers slightly while it increased the number of basal and lateral breaks, number of buds and chlorophyll content. Increasing Ca concentrations increased the number of buds and shoot dry weight, while Ca concentrations of 150 mg/l stimulated the greatest number of branches, within 3cm of the base of the plant, and chlorophyll content. Shoot N and Ca concentrations of from 3.2 to 5.1% (dw) basis and 0.8 to 1.2% (dw) respectively was associated with good plant growth and flower production. In a second N experiment, a soluble, commercially available fertilizer (20–8.8–16.6, N‐P‐K) was applied to plants growing in a peat, vermiculite, sand, perlite medium with fertilizer amendments. Nitrogen concentrations from 100 to 1900 mg/l were applied weekly. It was found that a weekly application of from 1000 to 1500 mg N/l from a soluble complete fertilizer maximized flowering and growth.