Gentamicin is the only antibiotic currently added to commercially available corneal storage media. To reduce the potential for bacterial dissemination from donor corneal tissue to the recipient eye, we evaluated the addition of vancomycin to corneal storage media. When added to Dexsol at a concentration of 200 μ,g/ml, vancomycin levels were maintained, showing at 7 % decrease in vancomycin concentration per month, measured== 90 days after its addition. Human corneas were stored in gentamicin-free Dexsol (Chiron Ophthalmics, Inc., Irvine, CA, U.S.A.) containing 150 μ g/ml vancomycin. Corneal tissue levels of vancomycin determined by agar diffusion bioassay were 201, 226, 292 μ g/ml at 1, 3, and 7 days of storage respectively, suggesting that corneal tissue concentrates vancomycin with time. No differences in endothelial cell count or cell death were seen in corneas stored in Dexsol (containing gentamicin) or Dexsol plus vancomycin when followed for *sl4 days. Vancomycin added to corneal storage media should reduce the potential for endophthalmitis due to gentamicin-resistant organisms.