Simple Thin Layer Chromatography Method with Fibre Optic Remote Sensor for Fluorimetric Quantification of Tryptophan and Related Metabolites
作者:
R. Linares Aponte,
J. Ayala Díaz,
A. Afonso Perera,
V. González Díaz,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 5
页码: 687-698
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608005530
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Tryptophan (TP), 5-hydroxytryptophan (5-HTP), 3-indoleacetic acid (IAA) and serotonin (5-HT) were separated by TLC, with chloroform-methanol-ammonia (12-7-1) (v-v-v) as eluent and cellulose as stationary phase. A fibre optic-based fluorescence instrument for in situ scanning was used for quantitative measurements. The compounds were determined over the range 10–100 ng, with relative standard deviations between 1.70–6.52% and detection limits over the range 16.39–22.50 ng.
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