Induction of exocytosis in characean internodal cells by locally restricted application of chlortetracycline and the effect of cytochalasin B, depolarizing and hyperpolarizing agents*
作者:
I. FOISSNER,
期刊:
Plant, Cell&Environment
(WILEY Available online 1991)
卷期:
Volume 14,
issue 9
页码: 907-915
ISSN:0140-7791
年代: 1991
DOI:10.1111/j.1365-3040.1991.tb00959.x
出版商: Blackwell Publishing Ltd
关键词: Chara corallina;Characcae;exocytosis;Ca2+;chlortetra‐cycline;cytoskeleton;membrane potential;Sephadex;cell wall
数据来源: WILEY
摘要:
Abstract.Local exocytosis with concomitant deposition of cell wall material (plugs) was induced in mature characean internodal cells by application of Sephadex beads loaded with a chlortetracycline (CTC)/CaCl2solution. Plugs can be formed anywhere on the cells independently of structural and/or functional plasma membrane domains, provided that the pH of the CTC/CaCl2solution is above 5.5. Plug formation is inhibited by cytochalasin B and membrane depolarizing agents. Treatment with fusicoccin enhances exocytosis. The results suggest that actin filaments are involved in the transport of vesicles and endoplasmic reticulum cisternae towards the cell membrane, and that the membrane potential determines the extent of CTC mediated Ca2+influx.
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