HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION AND QUANTITATION OF STEVIOSIDE AND ITS METABOLITES
作者:
A.M. Hutapea,
C. Toskulkao,
P. Wilairat,
D. Buddhasukh,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 8
页码: 1161-1170
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101724
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A method of analysis using high performance liquid chromatography (HPLC) was developed for the separation and quantitation of the metabolites of stevioside: steviol-16,17α-epoxide, 15α-hydroxysteviol, steviolbioside, isosteviol, and steviol. The separation was carried out on a reversed-phase C18 Nova-Pack column with gradient elution of acetonitrile/water mixture. The applicability of the method was demonstrated in the detection and separation of stevioside and its metabolites found in blood, feces, and urine of hamsters after ingestion of stevioside.
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