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Preparation and characterization of a monoclonal antibody specific for the β‐agonist clenbuterol

 

作者: Eleonora Petruzzelli,   Adriano Ius,   Silvia Berta,   Mauro Dovis,   Alberto Albertini,  

 

期刊: Food and Agricultural Immunology  （Taylor Available online 1996）
卷期: Volume 8, issue 1  

页码: 3-10

 

ISSN:0954-0105

 

年代: 1996

 

DOI:10.1080/09540109609354898

 

出版商: Taylor & Francis Group

 

关键词: ELISA;monoclonal antibodies;ß‐agonists;clenbuterol;urine

 

数据来源: Taylor

 

摘要:

A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by γ1,κ isotype and an affinity constant of 3.5 × 108l mol‐1, is highly specific as its cross‐reactivity to structurally related molecules is less than 1%. A competitive ELISA for the detection of clenbuterol in horse and human urine was developed using this antibody.

 

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