New Techniques for Qligosaccharide Sequencing
作者:
VernonN. Reinhold,
Eric Coles,
StevenA. Carr,
期刊:
Journal of Carbohydrate Chemistry
(Taylor Available online 1983)
卷期:
Volume 2,
issue 1
页码: 1-18
ISSN:0732-8303
年代: 1983
DOI:10.1080/07328308308058806
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new procedure is described for oligosaccharide sequencing that combines HPLC purification, the sensitive technique of fluorescence detection, and partial polymer degradation by ionic hydrogenation with component determination by direct chemical ionization mass spectrometry (DCI-MS) and GC-MS. The methodology is demonstrated for a series of model compounds and is shown to provide linkage information as well as sugar sequence.
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