Samejima M, Happe HK, Murrin LC, Pfeiffer RE, Ebadi M. Distribution of cholinergic and dopaminergic receptors in rainbow trout pineal gland. J. Pineal Res. 1994: 16: 37–43.AbstractThe involvement of multiple receptors in modulating the function of the pineal gland was investigated by searching for dopaminergic and cholinergic receptors in trout pineal gland. Dopamine D1and D2receptors were measured using [3H]SCH23390 and [3H]spiperone, respectively. Muscarinic and nicotinic cholinergic receptors were measured using quinuclidinyl benzilate ([3H]QNB) and [3H]methylcarbamyl choline, respectively. High‐affinity choline uptake sites were measured using [3H]hemicholinium‐3. The distribution of dopaminergic receptors varied throughout the pineal gland in that the density of D2receptors, which was higher than that of D1receptors, was most abundant in the distal region, exhibiting a value of 112 ± 17 fmol/mg tissue. The distribution of both muscarinic and nicotinic receptors was uniform throughout the pineal gland. However, the highest value for the high‐affinity choline transporter (106 ± 17 fmol/mg tissue) occurred in the proximal portion of the trout pineal gland. The results of these studies indicate that the pineal gland should not be viewed as a homogenous tissue possessing identical density of various receptors. Furthermore, these results, along with previous data, are interpreted to suggest that different regions of pineal gland may indeed possess unique