Nicotinic acetylcholine subunit mRNA expression in dopaminergic neurons of the rat substantia nigra and ventral tegmental area
作者:
Eric Charpantier,
Pascal Barnéoud,
Paul Moser,
François Besnard,
Frédéric Sgard,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 13
页码: 3097-3101
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Dopaminergic;Nicotinic acetylcholine receptors;RT-PCR;Substantia nigra;Ventral tegmental area
数据来源: OVID
摘要:
THE molecular composition of the nicotinic acetylcholine receptors (nAChRs) located on dopaminergic neurons and modulating their activity is unclear. Using the reverse transcriptase-polymerase chain reaction we have analyzed the mRNA for nAChR subunits expressed in the substantia nigra (SN) and ventral tegmental area (VTA) following unilateral 6-hydroxy-dopamine lesion of the dopaminergic system. In contrast to the unlesioned hemisphere, no signal was found in the lesioned hemisphere for α3, α5, alpha;6, and β4 subunits in the SN nor for α2, α3, α5, α6, α7 and β4 subunits in the VTA, indicating the expression of these subunits in dopaminergic neurons. mRNA for α4, β2 and β3 subunits (and α7 in the SN) were still detected after lesion, suggesting that they are expressed in GABAergic neurons and interneurons of these brain areas. These results demonstrate the selective localisation of a number of nAChR subunit mRNA within dopaminergic neurons, strongly suggesting that a heterogenous population of nAChRs play a role in modulating dopaminergic neuronal activity.
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