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Operator-assisted semi-automatic karyotyping of banded metaphases

 

作者: H.C. Wulf,  

 

期刊: Cytogenetic and Genome Research  (Karger Available online 1977)
卷期: Volume 19, issue 2-3  

页码: 146-158

 

ISSN:1424-8581

 

年代: 1977

 

DOI:10.1159/000130805

 

出版商: S. Karger AG

 

数据来源: Karger

 

摘要:

Computerized chromosome analyses have resulted in up to 25 % erroneous classifications, whereas a cytogenetically trained laboratory technician has an error rate of less than 0.1 % in the preparation of a karyotype. In the project reported here, computerized classification is eliminated, and, instead, the other steps of the analysis leading to the final karyotype are automated. In the instrument is a projection microscope, modified to project the image on paper before the operator. The slide is scanned on a motor-driven scanning table, in search of a suitable metaphase. The operator stops the scanning motor when a suitable metaphase comes into view. The individual chromosomes of the metaphase are then framed, using a view finder, and are identified, after which the chromosome number is entered on a keyboard. The instrument automatically ensures that, for each data entry, the framed chromosome is photographed in such a manner that the chromosome is placed in the proper location with the correct orientation in the karyotype. When all chromosomes have been identified, and the data have been keyed in, a photograph of the finished karyotype is presented. Marking the metaphase chromosomes on the paper, chromosomes are counted within 20 s. With conventional methods, a total chromosome analysis takes 3–6 h, but the above-described device requires 20–30

 

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