Separation and Determination of Auxins by Capillary Electrophoresis
作者:
J. Liu,
S.F. Y. Li,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 11
页码: 1697-1713
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608013998
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
In this paper, the separation of fifteen plant auxins by capillary electrophoresis was investigated. A simple and efficient method was developed. The migration behaviour of the auxins under different separation conditions was studied. Optimum separation was obtained with cyclodextrin-modified borate-phosphate buffer. This method was applied to the determination of indole acetic acid (1AA), a well known auxin in plant samples, with cinnamic acid as the internal standard. The method is linear over the range of 0.4–8.0 mg/mL, with 82.4% recovery and a limit of quantitation of 0.4 mg/mL.
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